Related Experiment Video
Updated: Aug 20, 2026

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
An auxin-responsive SCARECROW-like transcriptional activator interacts with histone deacetylase
Ming-Jun Gao1, Isobel Parkin, Derek Lydiate
1Molecular Genetics Section, Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Canada.
Abstract:
Members of the plant-specific GRAS family of putative transcription factors are involved in various aspects of plant development. SCARECROW (SCR) is a member of this protein family and plays a significant role in the radial patterning of both roots and shoots. However, little is known about the regulation of SCR expression and its mode of action in plants. Here, we report on the isolation and characterization of a Brassica napus SCARECROW-like protein, BnSCL1, isolated by selecting for proteins that interact with the Arabidopsis histone deacetylase AtHDA19 in a yeast two-hybrid screen. BnSCL1 contains domains conserved in the GRAS family of proteins, interacts with AtHDA19 through a VHIID domain, and exerts transcription activation of reporter genes . BnSCL1 is expressed predominantly in the roots, where its expression is regulated by auxin, as it also is in shoots and mature leaves. These results indicate that BnSCL1 is a member of the GRAS family, and suggest that its mode of action in plant auxin response may involve interaction with HDA19.
Related Concept Videos
Co-activators and Co-repressors
Co-activators and Co-repressors
Histone Modification
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Histone Modification
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Chromatin Modification in iPS Cells
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

