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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase
Sylvain Precigou1, Marco Wieser, Patrick Pommares
1Laboratoire d'Ecologie Moléculaire, IBEAS, EA 3525, Université de Pau et des Pays de l'Adour, avenue de l'Université, UFR Sciences et Techniques, BP 1155 F-64013, Pau cedex, France.
Abstract:
Rhodococcus pyridinovorans MW3 was isolated from an arable land of manioc from the Congo for its ability to transform acrylonitrile to acrylamide. This strain contains a cobalt nitrile hydratase (NHase) showing high sequence homology with NHases so far described. The specific NHase activity was 97 U mg(-1) dry wt. NHase production by R. pyridinovorans MW3 was urea and Co-dependent. The NHase was active for acrylamide up to 60% (w/v) indicating its potential for acrylamide production.
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