Related Experiment Video
Updated: Aug 20, 2026
Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
Published on: September 16, 2021
[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes]
Xue-yong Cai1, Gui-an Chen, Ying Lian
1Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100083, China.
Objective:
To investigate the influence of cryoprotectants and cooling rates in vitrification method on the spindles of rabbit M II oocytes.
Methods:
Rabbit oocytes were verified by using cryoloop with ethylene glycol (EG) singly or EG combined with dimethyl sulphoxide (DMSO) as cryoprotectants, and cooled by taking oocytes directly into liquid nitrogen or by vitrification machine. After frozen rabbit oocytes thawed, the microtubulin and chromosome of the spindles were fixation and stained by immunofluorescent method. Confocal microscope was used to reveal spindle configuration.
Results:
In the two protocols of single EG used and EG combined with DMSO, the spindles were severely injured. But in protocol of EG combined with DMSO and at ultra-rapid cooling rate, the normal configuration of spindle rate of thawed rabbit oocytes was similar to that of the control group.
Conclusion:
The protocol of EG combined with DMSO as cryoprotectants and with extremely high cooling rate by vitrification machine can produce the best effect on conservation of spindle configuration in vitrification of rabbit oocytes.
