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A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated
Rama Garimella1, Joseph B Sipe, H Clarke Anderson
1Department of Pathology and Laboratory Medicine, University of Kansas Medical Center. Kansas City, KS-66160. USA.
Abstract:
A simple and non-radioactive technique based on O-cresolpthalein complexone assay was developed to study in vitro non-radioactive calcium ((40)Ca) deposition by isolated matrix vesicles. Using this technique, the effect of various phosphoester substrates including ATP, AMP and beta-GP on in vitro MV-calcification was studied. O-cresolpthalein complexone assay with non-radioactive calcium demonstrated that AMP or beta-GP were more effective in promoting calcium deposition by isolated MVs than ATP. The application of this non-radioactive technique, which is highly sensitive and simple, would offer a useful alternative approach to the routinely used radiometric biomineralization assay which employs radioactive (45)Ca.
