Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential structural properties and expression patterns suggest functional significance for multiple mouse

Donna Brennan1, Ying Hu, Ana Kljuic

  • 1Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, PA, USA.

Differentiation; Research in Biological Diversity
|December 21, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting extracellular collagen assembly to enhance functional recovery in composite neuromuscular trauma.

Connective tissue research·2026
Same author

Do Alopecia Areata and Hair Colour Have a Shared Genetic Component?

Experimental dermatology·2026
Same author

Multicytokine-based transcriptome-wide association study for 7 inflammatory skin disorders identifies candidate causal genes in keratinocytes.

The Journal of allergy and clinical immunology·2026
Same author

Development of a Humanized Anti-Fibrotic Antibody Targeting Extracellular Collagen Assembly to Reduce Post-Traumatic Scarring.

bioRxiv : the preprint server for biology·2026
Same author

DSG2-Directed CAR-T Cells Safely and Universally Eliminate Solid Tumors.

Research square·2026
Same author

Optical photothermal infrared spectroscopic assessment of microplastics in tissue models and non-digested human tissue sections.

The Analyst·2026

Mouse genomes possess multiple desmoglein 1 (Dsg1) genes, unlike humans. These novel Dsg1 isoforms exhibit distinct expression patterns and functions, particularly in skin and hair follicle development, suggesting evolutionary adaptation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genomics

Background:

  • Desmogleins (Dsg1-4) are crucial desmosomal cadherins in epithelial tissues.
  • Human genome has one Dsg1 gene, but mice possess multiple isoforms (Dsg1-alpha, -beta, -gamma).
  • Isoform-specific sequence divergence, particularly in extracellular anchoring (EA) domains, suggests functional differences.

Purpose of the Study:

  • To characterize the novel mouse Dsg1 isoforms (Dsg1-beta and -gamma).
  • To investigate the tissue-specific expression patterns of Dsg1 isoforms.
  • To explore the functional implications of Dsg1 isoform diversity in mouse development and evolution.

Main Methods:

  • Bioinformatic analysis of Dsg1 isoform sequences, focusing on EA domains.
  • Generation and validation of isoform-specific antibodies.

Related Experiment Videos

  • Immunohistochemical analysis of Dsg1 isoform expression in various mouse tissues (epidermis, hair follicle, sebaceous gland, stomach).
  • Assessment of Dsg1 isoform susceptibility to proteolytic cleavage by exfoliative toxin A.

    • Main Results:

    • Dsg1-beta and -gamma genes were identified flanking the original Dsg1-alpha gene.
    • Computational analysis indicated lower hydrophilic potential for Dsg1-gamma EA domain, suggesting stronger membrane affinity.
    • All Dsg1 isoforms showed differential expression in epidermal differentiating layers and hair follicle development.
    • Dsg1-beta and -gamma were found in sebaceous glands and stomach epithelium, while Dsg1-alpha was not.
    • Dsg1-alpha and Dsg1-beta, but not Dsg1-gamma, were cleaved by exfoliative toxin A.

    Conclusions:

    • Multiple Dsg1 genes in mice likely evolved to support complex tissue development, particularly in skin and hair.
    • Differential expression and functional properties of Dsg1 isoforms contribute to tissue-specific adhesion and responses.
    • The distinct cleavage patterns suggest varied roles in pathogen interactions or tissue remodeling.