Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regulation of STAT signalling by proteolytic processing.

Lisa Hendry1, Susan John

  • 1Peter Gorer Department of Immunobiology, Programme in Infection and Immunity, King's College, London, UK.

European Journal of Biochemistry
|December 21, 2004
PubMed
Summary

Proteolytic processing generates STATgamma proteins, which act as dominant-negative regulators of STAT signaling. Aberrant STATgamma expression is linked to diseases like leukemia, highlighting its role in biological regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TNF-α induces type I IFN signalling to suppress neurogenesis and recruit T cells.

Nature communications·2026
Same author

Asthma remission with benralizumab therapy in severe eosinophilic Indian asthmatics.

Lung India : official organ of Indian Chest Society·2026
Same author

A Prospective Observational Study Evaluating the PRogression Of BronchiEctasis in COPD Patients - Results from the PROBE - COPD Study.

International journal of chronic obstructive pulmonary disease·2026
Same author

Diagnosis of acute appendicitis at a pediatric emergency department within a general hospital.

Emergency radiology·2026
Same author

Prevalence of alloantibodies among antenatal women attending a teaching hospital in central Kerala.

Journal of family medicine and primary care·2025
Same author

The prevalence of vitamin D deficiency and its association to modifiable risk factors of cardiovascular disease among apparently healthy adults in Central Kerala.

Journal of family medicine and primary care·2025

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Biochemistry

Background:

  • Signal transducer and activator of transcription (STAT) proteins are crucial for cell proliferation, differentiation, and survival.
  • STAT protein activity is tightly regulated by various mechanisms, including proteolytic processing.
  • Proteolytic cleavage generates C-terminally truncated STATgamma proteins, which function as dominant-negative regulators.

Purpose of the Study:

  • To explore the role of proteolytic processing in STAT protein regulation.
  • To investigate the generation and function of STATgamma isoforms.
  • To understand the implications of STATgamma in disease pathology.

Main Methods:

  • Analysis of STAT protein cleavage by proteases.
  • Identification of STATgamma isoforms in different cellular contexts.

Related Experiment Videos

  • Investigation of STATgamma expression in disease patients.

    • Main Results:

    • STATgamma isoforms, lacking the transactivation domain, were identified for Stat3, Stat5a, Stat5b, and Stat6.
    • Evidence suggests serine proteases are involved in STAT proteolytic processing, with calpain also implicated.
    • Aberrant expression of Stat3gamma and Stat5gamma is observed in acute myeloid leukemia and cutaneous T-cell lymphoma.

    Conclusions:

    • Proteolytic processing is a significant regulatory mechanism for STAT protein activity.
    • STATgamma proteins act as dominant-negative regulators, impacting cellular processes.
    • Dysregulation of STATgamma contributes to the pathogenesis of certain cancers, indicating therapeutic potential.