Related Experiment Video
Updated: Aug 20, 2026

Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
Published on: November 4, 2015
Bovine viral diarrhoea virus is internalized by clathrin-dependent receptor-mediated endocytosis
B Grummer1, S Grotha, I Greiser-Wilke
1Department of Infectious Diseases, Institute for Virology, University of Veterinary Medicine Hannover, Foundation Buenteweg 17, 30559 Hannover, Germany. beatrice.grummer@tiho-hannover.de
Abstract:
Bovine viral diarrhoea virus (BVDV) is a pestivirus within the family Flaviviridae. In contrast to the members of the genus flavivirus, nothing is known about the viral entry route for pestiviruses. In this study, the process of BVDV infection following attachment to the cell surface was examined. BVDV clearly co-localizes with clathrin, with early endosome antigen-1 (EEA-1), an early endosome marker, and also with lysosomal-associated membrane protein-2 (LAMP-2), a lysosomal marker. BVDV internalization is inhibited by compounds that block clathrin- but not caveolae-dependent endocytosis. These findings demonstrate that BVDV enters the cells via the clathrin-coated pit pathway.
Related Concept Videos
Receptor-mediated Endocytosis
Receptor-mediated Endocytosis
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Receptor-Mediated Endocytosis
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Clathrin Coated Vesicles
Intralumenal Vesicles and Multivesicular Bodies
Intracellular Movement of Viruses and Bacteria

