Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pseudo-thermosetting chitosan hydrogels for biomedical application.

J Berger1, M Reist, A Chenite

  • 1School of Pharmacy, University of Lausanne, 1015 Lausanne, Switzerland.

International Journal of Pharmaceutics
|December 21, 2004
PubMed
Summary

Transparent chitosan/beta-glycerophosphate (betaGP) hydrogels require specific deacetylation degrees (DD). Homogeneously reacetylated chitosan with a DD between 35-50% is key for transparency.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrastructure of hybrid chitosan-glycerol phosphate blood clots by environmental scanning electron microscopy.

Microscopy research and technique·2007
Same author

Cytocompatible gel formation of chitosan-glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal.

Journal of biomedical materials research. Part A·2007
Same author

Pseudo-thermosetting chitosan hydrogels for biomedical application.

International journal of pharmaceutics·2004
Same author

Effects of steam sterilization on thermogelling chitosan-based gels.

Journal of biomedical materials research·2001
Same author

Rolipram does not normalize very long-chain fatty acid levels in adrenoleukodystrophy protein-deficient fibroblasts and mice.

Journal of inherited metabolic disease·2000
Same author

New insights into p53 regulation and gene therapy for cancer.

Biochemical pharmacology·2000

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry

Context:

  • Chitosan-based hydrogels are promising biomaterials.
  • Achieving transparency in chitosan/beta-glycerophosphate (betaGP) hydrogels is challenging.
  • Controlling the deacetylation degree (DD) of chitosan is crucial for material properties.

Purpose:

  • To investigate methods for preparing transparent chitosan/betaGP pseudo-thermosetting hydrogels.
  • To optimize the reacetylation process of chitosan for improved hydrogel transparency.
  • To establish the relationship between chitosan DD, reacetylation homogeneity, and resulting hydrogel turbidity.

Summary:

  • Two reacetylation methods (I and II) were compared, with Method II demonstrating improved efficiency and reproducibility.
  • Chitosan with DDs from 35.0% to 83.2% was prepared using Method II under varying homogeneity conditions.

Related Experiment Videos

  • Hydrogel turbidity was found to be dependent on chitosan DD and the homogeneity of the reacetylation medium, influencing monomer distribution.
  • Transparent chitosan/betaGP hydrogels necessitate homogeneously reacetylated chitosan with a DD range of 35-50%.

    • Impact:

    • Provides a reproducible method for preparing transparent chitosan/betaGP hydrogels.
    • Identifies optimal conditions for chitosan modification to achieve desired optical properties.
    • Offers insights into controlling hydrogel characteristics through precise manipulation of polymer structure and processing.