Related Experiment Video
Updated: Aug 20, 2026

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
Published on: December 25, 2021
The study of the effects of low-level exposure to ionizing radiation using a bio-indicator system
Teresa Cristina do Santos1, V R Crispim, Y Noualhetas
1Programma de Engenharia Nuclear/COPPE/UFRJ, Rio de Janeiro, Brazil.
Abstract:
The object of the present study was to apply the high sensitivity of a botanical mutagenicity test (Tradescantia stamen-hair mutation bioassay), in "in situ" bioassays to determine the responses induced by the exposure to low levels of ionizing radiation. Mutagenesis was evaluated during 28 days, in an environment that presented gamma radiation exposure rates of 1.6 (control), 25.0 and 75.0 microR min(-1). From the results we observed that there was no linear response throughout the exposure time when compared mutagenic events and gamma radiation exposure rates were compared. The results obtained in the second week of exposure of Tradescantia showed that after exposure to 25.0 and 75.0 microR min(-1) there was a significant increase (p<0.05) in the mutation levels in Tradescantia stamen-hair. In the third and fourth week after exposure 25.0microR min(-1), the plants demonstrated a mutation rate that was not significantly different from the control (p>0.05).
Related Concept Videos
Biological Effects of Radiation
Toxicity Testing in Animals
Imaging Studies II: Positron Emission Tomography and Scintigraphy
Fundamental Principles of PET
Isotopes and Radioisotopes
An isotope containing more...

