REBASE--restriction enzymes and DNA methyltransferases

Area of Science:

• Molecular Biology
• Genomics
• Bioinformatics

Background:

• Restriction-modification systems are crucial for microbial defense and genome regulation.
• Accurate and up-to-date information on these systems is essential for molecular biology research.
• REBASE serves as a central repository for data on restriction enzymes and related proteins.

Purpose of the Study:

• To maintain and update REBASE, a comprehensive database of restriction enzymes, DNA methyltransferases, and related proteins.
• To provide researchers with detailed information on recognition sites, cleavage specificities, and commercial availability.
• To analyze and present data on restriction-modification systems in sequenced bacterial and archaeal genomes.

Main Methods:

• Compilation of published and unpublished experimental data.
• Inclusion of sequence and crystal structure data.
• Bioinformatic analysis of predicted restriction-modification systems in genomic data.

Main Results:

• REBASE contains extensive information on restriction enzymes, DNA methyltransferases, and homing endonucleases.
• Detailed data on recognition/cleavage sites, isoschizomers, and methylation sensitivity is available.
• Analysis of restriction-modification systems in sequenced prokaryotic genomes is integrated.

Conclusions:

• REBASE is an indispensable, continuously updated resource for researchers in molecular biology, genomics, and biotechnology.
• The database facilitates the study of DNA modification and restriction systems.
• Accessible via web and FTP, REBASE supports diverse research applications.