5'SAGE: 5'-end Serial Analysis of Gene Expression database
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Updated: Aug 20, 2026

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2
Published on: September 18, 2021
Yasuhiro Kasai1, Shin-ichi Hashimoto, Tomoyuki Yamada
1Department of Computational Biology, Graduate School of Frontier Science, The University of Tokyo, 5-1-5 Kashinoha, Kashiwa City, Chiba 277-8562, Japan.
Abstract:
To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5' end Serial Analysis of Gene Expression (SAGE) was developed recently, which makes it possible to collect a large amount of start site information, and subsequently, we have established a related database server called 5'SAGE. This database displays the observed frequencies of individual 5' end SAGE tags and previously unknown transcription start sites in the promoter regions, introns and intergenic regions of known genes. 5'SAGE will be useful for analyzing promoter regions and start site variation in different tissues, and is freely available at http://5sage.gi.k.u-tokyo.ac.jp/.
