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Protein open-access liquid chromatography/mass spectrometry
Wendy L White1, Craig D Wagner, John T Hall
1Discovery Research, GlaxoSmithKline, Research Triangle Park, NC 27709, USA.
Rapid Communications in Mass Spectrometry : RCM
|December 21, 2004
Summary
This study introduces protein open-access (OA) LC/MS, a fully automated system for protein characterization. It significantly reduces manual work in routine liquid chromatography/mass spectrometry analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Proteomics
Background:
- Increasing demand for routine protein characterization via liquid chromatography/mass spectrometry (LC/MS).
- Need for reduced analyst intervention in high-throughput protein analysis.
Purpose of the Study:
- To develop and implement a fully automated open-access LC/MS system for protein characterization.
- To streamline the process of protein analysis, from sample submission to report generation.
Main Methods:
- Development of a protein open-access (OA) LC/MS system.
- Web-enabled sample submission and registration.
- Automated data processing, interpretation, and report generation using LIMS and Oracle database.
- Utilizing protein sequence for theoretical molecular weight calculation and spectral analysis.
Main Results:
- Successful implementation of a fully automated LC/MS workflow.
- Significant reduction in analyst intervention for routine protein characterization.
- Deployment of three protein OA-LC/MS instruments supporting various protein-related efforts.
Conclusions:
- The developed protein OA-LC/MS system effectively automates routine protein characterization.
- This automation enhances efficiency and throughput in protein analysis.
- The system supports protein characterization, purification, and modification studies.