Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coronavirus transcription: a perspective.

S G Sawicki1, D L Sawicki

  • 1Department of Microbiology, Medical College of Ohio, Toledo, OH 43614, USA. ssawicki@mco.edu

Current Topics in Microbiology and Immunology
|December 22, 2004
PubMed
Summary

A new model explains coronavirus transcription, proposing discontinuous RNA synthesis occurs during minus-strand production. This clarifies how subgenomic mRNAs are templated from the genome, not from subgenomic mRNAs themselves.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatography of mouse hepatitis virus replicative intermediate and replicative form RNA.

Advances in experimental medicine and biology·2002
Same author

Mouse hepatitis virus minus-strand templates are unstable and turnover during viral replication.

Advances in experimental medicine and biology·2002
Same author

Role of the nonstructural polyproteins in alphavirus RNA synthesis.

Advances in experimental medicine and biology·1998
Same author

A new model for coronavirus transcription.

Advances in experimental medicine and biology·1998
Same author

Negative strand RNA synthesis by temperature-sensitive mutants of mouse hepatitis virus.

Advances in experimental medicine and biology·1998
Same author

Selection in persistently infected murine cells of an MHV-A59 variant with extended host range.

Advances in experimental medicine and biology·1998

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Established models for coronavirus transcription, like leader-primed transcription, were based on incomplete evidence regarding negative-strand RNA presence.
  • The discovery of subgenome-length minus strands necessitated revised models for coronavirus RNA synthesis.

Purpose of the Study:

  • To present a novel model for coronavirus transcription that explains the generation of subgenome-length minus strands.
  • To review experimental evidence supporting a new mechanism for discontinuous RNA synthesis in coronaviruses.

Main Methods:

  • Review of experimental evidence presented at the VIth International Symposium on Corona and Related Viruses.
  • Comparative analysis of existing and proposed models for coronavirus RNA synthesis.

Main Results:

  • The previously accepted leader-primed transcription model was based on erroneous data.
  • The replicon model proposed subgenomic mRNAs as initial products later replicated into templates.
  • A new model posits discontinuous RNA synthesis occurs specifically during minus-strand synthesis from the genome.

Conclusions:

  • Coronavirus genome RNA is copied both continuously and discontinuously to produce minus-strand templates.
  • Minus-strand templates are generated for both genome RNA synthesis and subgenomic mRNA synthesis.
  • Subgenomic mRNAs do not serve as templates for minus-strand synthesis; only the genome does.

Related Experiment Videos