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Updated: Aug 20, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
C-type cytochrome formation: chemical and biological enigmas
Julie M Stevens1, Oliver Daltrop, James W A Allen
1Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
Abstract:
C-type cytochromes are proteins that are essential for the life of virtually all organisms. They characteristically contain heme that is covalently attached via thioether bonds to two cysteines in the protein. In this Account, we describe the challenging chemistry of thioether bond formation and the surprising variety of biogenesis systems that exist in nature to perform the difficult posttranslational heme attachment process. We show what insight has been gained into the various biogenesis systems from in vitro and in vivo experiments and highlight some forthcoming challenges in this field.
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