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Related Experiment Videos

Broad-based proteomic strategies: a practical guide to proteomics and functional screening.

David R M Graham1, Steven T Elliott, Jennifer E Van Eyk

  • 1Department of Medicine, Division of Cardiology, Johns Hopkins University, Baltimore, MD 21224, USA.

The Journal of Physiology
|December 22, 2004
PubMed
Summary

This review guides broad-based proteomics projects, focusing on selecting appropriate technologies for biological systems and hypotheses. It covers refining hypotheses, choosing models, and understanding proteomic technologies for disease research.

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Area of Science:

  • Proteomics and its application in biological and medical research.

Background:

  • Proteomics, the study of proteins, is a rapidly advancing field with numerous technologies.
  • The high cost and complexity of proteomic technologies necessitate careful selection for specific research questions.

Purpose of the Study:

  • To provide guidelines for broad-based proteomics projects.
  • To assist researchers in selecting appropriate proteomic technologies based on biological systems and hypotheses.
  • To discuss strategies for hypothesis refinement, model selection, and technology choice.

Main Methods:

  • Review of various proteomic technologies and their applications.
  • Emphasis on sample complexity, including protein abundance and characteristics.
  • Discussion of separation technologies, quantification, mass spectrometry, and informatics strategies.

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Main Results:

  • Outlines proteomic technologies applicable to broad-based approaches.
  • Details the application of these technologies in physiological and pathological processes.
  • Highlights the importance of integrating research design with advanced technologies.

Conclusions:

  • Effective proteomics requires careful planning and the integration of complementary techniques.
  • Powerful discovery technologies can significantly advance the understanding of diseases.
  • The strategic application of proteomic technologies is crucial for biological and medical breakthroughs.