Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Axially symmetric semi-infinite domain models of microdialysis and their application to the determination of

Jason L Roberts1, John M B Newman, Roland Warner

  • 1Tasmanian Partnership for Advanced Computing, University of Tasmania, Private Bag 37, Hobart 7001, Tasmania, Australia.

The Journal of Physiology
|December 22, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polygenic Risk Scores for Cardiovascular Disease: Closing the Gap Between Prediction and Practice.

Journal of the American College of Cardiology·2026
Same author

A sparse proteomic risk score incorporating plasma MMP12 level improves prediction of abdominal aortic aneurysm.

medRxiv : the preprint server for health sciences·2025
Same author

Seasonal variation of trifluoroacetic acid (TFA) in Antarctic ice cores analysed by direct injection non-suppressed ion chromatography-mass spectrometry (nsIC-MS).

The Science of the total environment·2025
Same author

Segmented linear integral correlation Kernel ensemble reconstruction: A new method for climate reconstructions with applications to Holocene era proxies from an East Antarctic ice core.

PloS one·2025
Same author

Bedmap3 updated ice bed, surface and thickness gridded datasets for Antarctica.

Scientific data·2025
Same author

Patient and Operative Factors Predict Risk of Discretionary Prolonged Postoperative Mechanical Ventilation in a Broad Surgical Cohort.

Anesthesia and analgesia·2023

New models using microdialysis can estimate muscle blood flow fractions. Noradrenaline increased nutritive blood flow, while serotonin decreased it, offering insights into microvascular conditions.

Area of Science:

  • Physiology
  • Biomedical Engineering
  • Pharmacology

Background:

  • Microdialysis is a technique used to measure substances in tissue fluid.
  • Understanding muscle blood flow is crucial for diagnosing and treating various conditions.
  • The nutritive fraction of blood flow is a key indicator of tissue perfusion.

Purpose of the Study:

  • To develop theoretical models for describing the microdialysis outflow:inflow (O/I) ratio.
  • To incorporate the nutritive fraction of total blood flow in muscle into these models.
  • To apply the models for estimating the nutritive fraction of skeletal muscle blood flow under different physiological conditions.

Main Methods:

  • Developed theoretical models for the O/I ratio using 3H2O and [14C]ethanol tracers.

Related Experiment Videos

  • The models considered probe dimensions, flow rate, muscle blood flow, and tracer diffusion coefficients.
  • Validated models against experimental data from constant-flow perfused rat hindlimb.

    • Main Results:

    • The models produced an exponential decay expression for the O/I ratio.
    • Basal nutritive fraction was estimated at 0.22 +/- 0.04.
    • Noradrenaline (70 nM) significantly increased nutritive fraction to 0.91 +/- 0.06.
    • Serotonin (300 nM) significantly decreased nutritive fraction to 0.05 +/- 0.01.

    Conclusions:

    • The developed microdialysis models accurately estimate the nutritive fraction of muscle blood flow.
    • Pharmacological agents like noradrenaline and serotonin significantly alter nutritive blood flow.
    • This model provides a valuable tool for assessing skeletal muscle microvascular function in various physiological and pathological states.