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Updated: Aug 20, 2026

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
[Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry used to bacteria detection]
Zongke Sun1, Wei Zhang, Xiping Chen
1Institute of Environment Hygiene and Health Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Abstract:
To investigate the influence variations, three culture media and different culture time were evaluated using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry for the rapid identification of eight strains of bacteria. These culture media are Colombia Blood Agar, Eosin Ethylene Blue Agar and Nutrition Agar. Results demonstrate that different culture media and culture time can influence the reproducibility of the bacteria fingerprint. It is suggested that appropriate culture media and culture time can enhance the accuracy of the bacteria detection using matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.
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