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Updated: Aug 13, 2026
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
[Expression of metallothioneins on zinc exposure in rat hippocampal neurons]
Haihong Qin1, Hui Shen, Fudi Wang
1Department of Military Hygiene, Second Military Medical University, Shanghai 200433, China.
Objective:
To study the homeostasis of zinc in central nervous system.
Methods:
Expression of MT1/MT2/MT3 on various concentration of zinc exposure (0-150 micromol/L) was determined and their expression on 100 micromol/L zinc exposure after 0, 2, 4, 6 and 8 hours in primary culture rat hippocampal neurons was measured by real-time fluorescent quantitative RT-PCR.
Results:
Amount of MT3 mRNA was highest followed by MT1 mRNA, MT2 mRNA ranked third. Expression of MT1 and MT2 mRNA could be up-regulated significantly on 75-100 micromol/L zinc exposure, and then, reach to a plateau on 125-150 micromol/L. Their peak mRNA levels appeared at about 6 h after addition of zinc was added. However, the MT3 mRNA was decreased by about 60% after zinc was added 6 h later.
Conclusion:
The results showed that not only the MT3 but also the MT1 and MT2 were involved in the regulation of zinc homeostasis in neurons, just like neuroglial cell do.
