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Updated: Aug 20, 2026
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
[Application of modified ABTS+ assay method to monitor antioxidant extraction from fruit]
Guangliang Han1, Cuimei Li, Eduardo Cacace
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Objective:
Modify the ABTS method to measure the antioxidant activity of compound. Apply this method to optimize antioxidant extraction from fruit.
Methods:
In microplate, Trolox served as reference. Antioxidant activity was measured according to their ability to scavenge ABTS+ in the reaction mixture. Under different temperatures, solvent solid ratios, solvent concentrations, antioxidants were extracted.
Results:
The standard error for measuring Trolox antioxidant activity was under 0.2. The antioxidant activities of fourteen standard samples were consistent with that reported in literatures. Using ethanol as solvent, at about 17 degrees C, for 1 gram of blackcurrant 20 ml of 67% ethanol was appropriate. While using sodium metabisulphite as solvent, at about 30 degrees C, for 1 gram of blackcurrant 20 ml of 300 mg/L metabisulphite would be appropriate.
Conclusion:
Modified ABTS+ method for measuring antioxidant activity was simple and feasible. This method could be applied to monitor antioxidant extraction from fruit and high throughput screening for antioxidant.
