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Updated: Aug 11, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Published on: October 15, 2013
[Single cell gel electrophoresis assay--applications and progress]
Caihong Xing1, Guilan Li, Songnian Yin
1Institute for Occupational Health and Poison Control, China CDC, Beijing 100050, China.
Abstract:
The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method for DNA double- and single-strand breaks and alkali-labile sites, in eukaryotic individual cells. In recent years, this method has been widely used for studies on DNA repair, genetic toxicology, environmental biomonitoring and apoptosis. In this paper, the progress of the assay in: (1) fluorescence in situ hybridization with comet, (2) the use of repair enzymes to detect specific classes of DNA damage, and (3) DNA-specific dye, slide storage and analysis of comet were reviewed.
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