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Related Experiment Videos

Retinol improves bovine embryonic development in vitro.

Tracy Livingston1, Dawn Eberhardt, J Lannett Edwards

  • 1Department of Animal Science, University of Tennessee, Knoxville, TN 37996, USA. Tracy_Livingston@georgetowncollege.edu <Tracy_Livingston@georgetowncollege.edu>

Reproductive Biology and Endocrinology : RB&E
|December 23, 2004
PubMed
Summary

Adding retinol during bovine oocyte maturation improved embryo development, particularly under atmospheric oxygen. This suggests retinol

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Area of Science:

  • Reproductive biology and developmental science.
  • Cellular differentiation and tissue function.
  • Vitamin A metabolism and its biological roles.

Background:

  • Retinoids are crucial for vertebrate development and reproductive processes.
  • Previous research indicates retinoids impact follicular development, oocyte maturation, and early embryonic development.
  • The specific effects of retinol on bovine oocyte maturation and early embryo development require further investigation.

Purpose of the Study:

  • To evaluate the in vitro effects of retinol on bovine oocyte maturation and subsequent embryonic development.
  • To compare the efficacy of retinol supplementation under low (7%) and atmospheric (20%) oxygen conditions.
  • To determine optimal conditions for retinol's influence on blastocyst formation rates.

Main Methods:

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  • Bovine oocytes were matured in vitro with varying retinol concentrations.
  • Oocytes were fertilized and cultured in modified synthetic oviductal fluid (mSOF) under controlled oxygen levels (7% or 20%).
  • Cleavage and blastocyst formation rates were assessed to evaluate embryonic development.

Main Results:

  • Retinol addition to maturation medium tended to increase blastocyst formation rates (p < 0.07).
  • 5 micromolar retinol significantly improved blastocyst development under 20% oxygen conditions (p < 0.001).
  • Retinol supplementation enhanced blastocyst/cleaved rates by nearly 10% (p < 0.02).

Conclusions:

  • Supplementation with 5 micromolar retinol during in vitro maturation may enhance bovine oocyte embryonic development.
  • Retinol demonstrates a beneficial effect on blastocyst development, especially under atmospheric oxygen, suggesting an antioxidant role.
  • Further research can explore optimizing retinol concentrations for improved assisted reproductive technologies in cattle.