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Related Experiment Videos

Dicer is required for embryonic angiogenesis during mouse development.

Wei J Yang1, Derek D Yang, Songqing Na

  • 1Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA.

The Journal of Biological Chemistry
|December 23, 2004
PubMed
Summary

Dicer is essential for mouse development, with mutations causing embryonic lethality. This study reveals Dicer

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Dicer protein processes double-stranded RNA into small interfering RNAs (siRNAs) and microRNAs (miRNAs).
  • miRNAs regulate gene expression and are involved in developmental timing.
  • The precise role of Dicer in mammalian development and angiogenesis remained unclear.

Purpose of the Study:

  • To investigate the essential role of Dicer in mammalian embryonic development.
  • To determine Dicer's function in angiogenesis.

Main Methods:

  • Generation of Dicerex1/2 mutant mice with a specific deletion in the Dicer gene.
  • Phenotypic analysis of homozygous mutant embryos.
  • Assessment of blood vessel formation and expression of angiogenesis-related genes.

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Main Results:

  • Dicerex1/2 homozygous embryos exhibited retarded development and embryonic lethality between 12.5 and 14.5 days of gestation.
  • Mutant embryos showed severely compromised blood vessel formation and maintenance in embryos and yolk sacs.
  • Expression levels of key angiogenic factors (VEGF, FLT1, KDR, TIE1) were altered in mutant embryos.

Conclusions:

  • Dicer is indispensable for normal mouse embryonic development.
  • Dicer plays a critical role in embryonic angiogenesis, likely through miRNA processing.
  • Dicer's function in angiogenesis may involve regulating key angiogenic factors.