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Updated: Aug 20, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Published on: January 30, 2018
Three- and four-way parallel factor (PARAFAC) analysis of photochemically induced excitation-emission kinetic
Michelle L Nahorniak1, Gary A Cooper, Yoon-Chang Kim
1Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA.
Abstract:
Independently emerging fluorescence profiles of unknown, photochemically induced degradation products of several naturally non-fluorescent pesticides were monitored using single exposure excitation-emission fluorescence spectroscopy. Three-way parallel factor analysis (PARAFAC) was employed to uniquely resolve the pure fluorescent spectra of the overlapping photolysis products. The quantitative utility of EEM photolysis-based determinations was demonstrated by employing four-way PARAFAC models built from EEM time cubes of multiple fenvalerate samples. The 4-way PARAFAC models were then used to predict original pesticide concentrations resulting in conservative limit of detection and root mean square errors of calibration (RMSEC) of 3 microM each.
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