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Related Experiment Videos

Cystatin C uptake in the eye.

Johan Wassélius1, Kjell Johansson, Katarina Håkansson

  • 1Department of Ophthalmology, University Hospital, Lund University, 221 85, Lund, Sweden.

Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
|December 23, 2004
PubMed
Summary

Cystatin C is actively taken up by cells in the eye, including the cornea and retina. This active uptake suggests cystatin C may function intracellularly and regulate extracellular levels.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biochemistry

Background:

  • Cystatin C is a secreted protein inhibiting cysteine proteases extracellularly.
  • Potential intracellular targets in the eye prompted investigation into cystatin C cellular uptake.

Purpose of the Study:

  • To investigate the cellular uptake of cystatin C in ocular tissues.
  • To determine if cystatin C can enter cells within the eye.

Main Methods:

  • Intravitreal injection of fluorescence-labeled cystatin C in rats.
  • Analysis of ocular tissues using ELISA, fluorescence microscopy, and immunohistochemistry.
  • In vitro study using a retina model to assess cystatin C uptake.

Main Results:

Related Experiment Videos

  • Cystatin C was taken up by corneal endothelium/epithelium, ciliary processes, neuroretina (ganglion cells), and retinal pigment epithelium.
  • Uptake was observed both in vivo and in vitro.
  • Neuroretinal uptake was high-affinity, temperature-dependent, and inhibited by cytochalasin D.

    • Conclusions:

    • An active, temperature-dependent uptake system for cystatin C exists in corneal, ciliary body, and retinal cells.
    • Intracellular cystatin C in the eye may originate from extracellular uptake.
    • This uptake mechanism suggests cystatin C may have intracellular functions and regulate extracellular levels.