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Emulsan quantitation by Nile red quenching fluorescence assay
Guillermo R Castro1, Bridget K Larson, Bruce Panilaitis
1Department of Biomedical Engineering, School of Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
Applied Microbiology and Biotechnology
|December 23, 2004
Abstract:
A Nile red fluorescent technique to quantify 20-200 microg ml(-1) of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm(-1) M(-1). Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.