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1D saturation transfer difference NMR experiments on living cells: the DC-SIGN/oligomannose interaction

Silvia Mari1, Diego Serrano-Gómez, F Javier Cañada

  • 1Centro de Investigaciones Biológicas, Departamentos de Inmunología y de Estructura de Proteínas, C.S.I.C., c/Ramiro de Maeztu 9, 28040 Madrid, Spain.

Angewandte Chemie (International Ed. in English)
|December 23, 2004
PubMed
Abstract

No abstract available in PubMed .

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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