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Cy3B: improving the performance of cyanine dyes
Michael Cooper1, Andreas Ebner, Mark Briggs
1Amersham Biosciences, Discovery Systems, The Maynard Centre, Whitchurch, Cardiff, CF14 7YT, United Kingdom. michael.cooper@amersham.com
Journal of Fluorescence
|December 24, 2004
Abstract:
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.