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Updated: Aug 20, 2026

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
Dynamic of the fluorescence of the complex Zn++/bis-N-carbazolyl-distyrylbenzene
Evgenia Vaganova1, Vladimir Khodorkovsky, Mark Sigalov
1Inorganic and Analytical Chemistry Department, The Hebrew University, Jerusalem, Israel.
Abstract:
The discrimination between similar concentrations of the different metal ions is one of the important roles of fluorescent sensors. Here we present the study of the fluorescence dynamic of the chromophore bis-N-carbazolyl-distyrylbenzene (BCDSB) in acetonitrile/water (mmol/L), doped with metal ions such as K+; Ca++; Mg++; Zn++(10 micromol/L). BCDSB has the fluorescence with lambda(max) at 448 nm by excitation at lambda(exc) = 378 nm, lifetime 1.089 ns: quantum yield of the fluorescence is 0.68. With continuation of irradiation fluorescence quenching has been registered for all investigated metal ions. However, in the presence of Zn++ oscillation of the intensity was observed. The energy activation of the oscillation as much as 15 kcal/mol was estimated. We believe, that the specificity of the complex Zn++/BCDSB, is in an asymmetrical structure, formed via binding sites of Zn++ with the electron-enriched binding sites of the BCDSB, excited in n(pi)* state. This asymmetrical complex structure can cause the photoinduced structural fluctuation in the complex coordination.
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