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Updated: Aug 20, 2026
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
[Determination of concentration and encapsulation efficiency of silybin liposomes]
Jiang-nan Yu1, Xi-ming Xu, Yuan Zhu
1School of Pharmacy, Jiangsu University, Zhenjiang 212001, Jiangsu, China.
Objective:
To determine the encapsulation efficiency of silybin liposomes.
Method:
After Silybin liposomes solution was separated through sphadex G-100 column, eluent collected was determined by the first derivative UV spectrometry.
Result:
The calibration curve of Silybin was linear in the range of 0.5-30.0 mg x L(-1) (r = 0.9998). The average recovery was 101.7%. The relative standard deviation was 1.8%. The results showed that the encapsulation efficiency ranged from 65.1%-83.0% when the drug/phospholipid ratio varied from 0.02-0.06. The concentration of SLB in liposomes prepared by optimum technique was 760.4 mg x L(-1) (n = 4).
Conclusion:
The method is accurate, simple and suitable for the quality control of Silybin liposomes.
