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The identification of a Clonorchis sinensis gene encoding an antigenic egg protein

Mejeong Lee1, Young-Bae Chung, Suk-Keun Lee

  • 1Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, 110-799, Seoul, Korea.

Parasitology Research
|December 24, 2004
PubMed

Insights

Researchers identified a Clonorchis sinensis egg protein, Cs28, which shows potential as a diagnostic antigen. This protein reacts with sera from clonorchiasis and other trematode infections, indicating its value in diagnosing parasitic diseases.

Area of Science:

  • Parasitology
  • Molecular Biology
  • Immunology

Background:

  • Clonorchis sinensis causes clonorchiasis, a significant public health issue in East Asia.
  • Antigenic proteins are crucial for developing diagnostic tools and understanding host-parasite interactions.

Purpose of the Study:

  • To screen the Clonorchis sinensis cDNA library for genes encoding antigenic proteins.
  • To characterize a novel antigenic protein, Cs28, and evaluate its diagnostic potential.

Main Methods:

  • cDNA library screening using sera from clonorchiasis patients.
  • Gene cloning and sequencing of the 888 bp gene encoding the 28-kDa Cs28 protein.
  • Immunohistochemical analysis of Cs28 in C. sinensis eggs.
  • Immunoblot analysis using recombinant Cs28 (rCs28) with sera from various parasitic infections.

Main Results:

  • The Cs28 protein, rich in glycine, tyrosine, and lysine, showed homology to vitelline proteins of other trematodes.
  • Immunohistochemistry confirmed Cs28 localization within C. sinensis intrauterine eggs.
  • rCs28 reacted with 73% of clonorchiasis patient sera and showed cross-reactivity with sera from opisthorchiasis, schistosomiasis, and paragonimiasis cases.

Conclusions:

  • The Cs28 protein is an egg antigen of Clonorchis sinensis.
  • Cs28 serves as a common antigen among several trematode species, suggesting its utility in diagnosing clonorchiasis and related helminth infections.

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