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Saccharide sensing using gold and silver nanoparticles--a review
Kadir Aslan1, Jian Zhang, Joseph R Lakowicz
1Center for Fluorescence Spectroscopy, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
Journal of Fluorescence
|December 25, 2004
Summary
New glucose sensing methods utilize nanoparticle interactions for tunable platforms. Concanavalin A (Con A) induces gold nanoparticle aggregation, while glucose causes dissociation, altering absorbance for detection.
Area of Science:
- Nanotechnology
- Biochemistry
- Analytical Chemistry
Background:
- Developing sensitive and specific glucose detection methods is crucial for managing diabetes.
- Existing glucose sensing technologies face challenges in sensitivity, selectivity, and cost-effectiveness.
- Nanoparticle-based biosensors offer potential for enhanced performance due to their unique optical and electronic properties.
Purpose of the Study:
- To present novel glucose sensing methodologies utilizing specific biological interactions and nanoparticle systems.
- To demonstrate tunable glucose sensing platforms based on dextran-coated gold and boronic-acid capped silver nanoparticles.
- To investigate the optical responses of these nanoparticle systems upon interaction with glucose.
Main Methods:
- Utilized dextran-coated gold nanoparticles and Concanavalin A (Con A) for aggregation-based sensing.
- Employed boronic-acid capped silver nanoparticles for luminescence-based glucose detection in solution.
- Applied transmission electron microscopy (TEM) to analyze nanoparticle aggregation.
- Utilized lifetime measurements to differentiate surface-enhanced fluorescence contributions.
Main Results:
- Con A induced aggregation of gold nanoparticles, increasing absorbance at 650 nm.
- Glucose addition caused gold nanoparticle aggregate dissociation, decreasing absorbance at 650 nm.
- Boronic-acid capped silver nanoparticles showed enhanced luminescence and altered absorbance (decrease at 400 nm, increase at 640 nm) in the presence of glucose and dextran.
- Surface-enhanced fluorescence was identified as a key sensing mechanism.
Conclusions:
- Developed novel, tunable glucose sensing platforms using distinct nanoparticle-based mechanisms.
- Demonstrated the potential of Con A-gold nanoparticle interactions for glucose detection.
- Validated the utility of boronic-acid capped silver nanoparticles for sensitive glucose quantification via luminescence.
- These approaches offer promising alternatives for glucose monitoring.