Advances in surface-enhanced fluorescence
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Updated: Aug 20, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Joseph R Lakowicz1, Chris D Geddes, Ignacy Gryczynski
1Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland, 725 West Lombard Street, Baltimore, Maryland 21201, USA. lakowicz@cfs.umbi.umd.edu
Abstract:
We report recent achievements in metal-enhanced fluorescence from our laboratory. Several fluorophore systems have been studied on metal particle-coated surfaces and in colloid suspensions. In particular, we describe a distance dependent enhancement on silver island films (SIFs), release of self-quenching of fluorescence near silver particles, and the applications of fluorescence enhancement near metalized surfaces to bioassays. We discuss a number of methods for various shaped silver particle deposition on surfaces.
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