Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology05:59

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

6.4K
An ultra-high-speed western blotting technique is developed by improving the kinetics of antigen-antibody binding through cyclic draining and replenishing (CDR) technology in conjunction with an immunoreaction enhancing...
6.4K
Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

4.4K
In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation...
4.4K
Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains12:28

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains

18.1K
This protocol describes a method to track individual brain-aging trajectories through a brain donation program and proper characterization of brains. Brain donors are involved in a long-term longitudinal study including serial multi-dimensional assessments. The protocol contains a detailed description of brain processing and an accurate diagnostic...
18.1K
Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting11:31

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

16.5K
We outline a methodology for the processing of whole blood to obtain a variety of components for further analysis. We have optimized a streamlined protocol that enables rapid, high-throughput simultaneous processing of whole blood samples in a non-clinical...
16.5K
Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies07:20

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

37.1K
Biomarkers are directly-measured biological indicators of disease or health. In population and social sciences, biomarkers need to be easy to obtain, transport, and analyze. Dried Blood Spot (DBS) collection meets this need, can be collected in the field with high response rates and analyzed for a variety of...
37.1K
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation10:43

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

42.7K
We are describing a new method of isolating genomic DNA from whole blood collected for plasma/serology. After plasma collection, the compacted blood is usually discarded. Our novel method represents a significant improvement over existing methods and makes DNA and plasma available from a single collection, without requesting additional...
42.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the slope: prognostic utility of the VE/VCO<sub>2</sub> intercept in chronic heart failure.

Open heart·2026
Same author

RoMa: A Cardiopulmonary Exercise Testing Based Risk Tool in Hypertrophic Cardiomyopathy.

Journal of the American Heart Association·2026
Same author

Method Validation of Cardiopulmonary Exercise Testing: Intra- and Inter-Device Comparisons Across Four Metabolic Carts.

European heart journal. Quality of care & clinical outcomes·2026
Same author

MECKI score thresholds for heart transplantation referral of ambulatory heart failure patients.

ESC heart failure·2026
Same author

Cardiopulmonary exercise test criteria for heart transplantation referral of patients with ambulatory heart failure in the current era.

Heart (British Cardiac Society)·2026
Same author

The need for a gender-specific HF approach, from prevention to cardiogenic shock.

Journal of cardiovascular medicine (Hagerstown, Md.)·2026

Related Experiment Video

Updated: Jan 5, 2026

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains
12:28

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains

Published on: June 3, 2020

18.1K

Spironolactone improves lung diffusion in chronic heart failure.

Piergiuseppe Agostoni1, Alessandra Magini, Daniele Andreini

  • 1Centro Cardiologico Monzino, IRCCS, Istituto di Cardiologia, Università di Milano, via Parea 4, 20138 Milan, Italy. piergiuseppe.agostoni@ccfm.it

European Heart Journal
|December 25, 2004
PubMed
Summary

Spironolactone treatment improved lung diffusion capacity (DLCO) and exercise capacity in chronic heart failure patients. This suggests a new way anti-aldosteronic drugs benefit heart failure (HF) outcomes.

More Related Videos

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
11:31

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

Published on: November 30, 2015

16.5K
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
10:43

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Published on: May 18, 2013

42.7K

Related Experiment Videos

Last Updated: Jan 5, 2026

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains
12:28

Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains

Published on: June 3, 2020

18.1K
Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
11:31

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

Published on: November 30, 2015

16.5K
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
10:43

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Published on: May 18, 2013

42.7K

Area of Science:

  • Cardiology
  • Pulmonology
  • Pharmacology

Background:

  • Chronic heart failure (HF) is associated with impaired lung diffusion (DLCO), linked to fibrosis.
  • Spironolactone, an anti-aldosteronic agent, improves HF prognosis and reduces connective tissue turnover.

Purpose of the Study:

  • To investigate the effect of spironolactone on DLCO in chronic HF patients.
  • To explore potential mechanisms of spironolactone's benefit in HF.

Main Methods:

  • Thirty stable chronic HF patients with reduced DLCO were randomized to spironolactone or placebo.
  • Evaluations included quality of life, laboratory tests, cardiopulmonary exercise tests, and pulmonary function tests (DLCO, DM) before and after 6 months.

Main Results:

  • Spironolactone significantly increased DLCO, primarily due to enhanced membrane diffusing capacity (DM).
  • Peak oxygen consumption (VO2) also increased significantly with spironolactone.
  • Improvements in DLCO and peak VO2 were strongly correlated.

Conclusions:

  • Spironolactone positively impacts gas diffusion and exercise capacity in chronic HF.
  • This suggests a novel mechanism involving improved lung function for spironolactone's benefits in HF.