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Adrenomedullin (AM) and receptor-activity-modifying proteins in glomeruli with Thy.1 glomerulonephritis

Michitaka Matsumoto1, Shouichi Fujimoto, Shuji Iwatsubo

  • 1First Department of Internal Medicine, Miyazaki Medical College, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki-gun, Miyazaki, 889-1692 Japan.

Clinical and Experimental Nephrology
|December 25, 2004
PubMed
Abstract

Insights

Adrenomedullin (AM) and its receptors (RAMPs) increased during the resolution of Thy.1 glomerulonephritis, not during peak proliferation. This suggests AM may help ameliorate kidney inflammation.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Immunology

Background:

  • Adrenomedullin (AM) exhibits antiproliferative and proapoptotic effects on mesangial cells in vitro.
  • Regulation of AM and its receptors in glomerulonephritis remains unclear.

Purpose of the Study:

  • To investigate the sequential changes in AM and its receptor (RAMP) expression and production in glomeruli during Thy.1 glomerulonephritis.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (RT-PCR) to measure mRNA expression.
  • Immunoradiometric assay (IRMA) to quantify AM protein levels.
  • Immunohistochemical staining to localize AM production.

Main Results:

  • AM and RAMP2 mRNA expression and AM protein levels were unchanged during the peak proliferation phase (day 7) of Thy.1 glomerulonephritis.
  • Significant increases in AM mRNA and protein were observed during the resolution phase (day 14).
  • AM production in mesangial cells was predominantly increased on day 14.

Conclusions:

  • Adrenomedullin (AM) and RAMPs increase during the resolution phase of Thy.1 glomerulonephritis, not during active proliferation.
  • The antiproliferative and proapoptotic actions of AM suggest a role in the amelioration of glomerulonephritis.

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