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Updated: Aug 20, 2026
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
Published on: April 3, 2017
[Isolation, culture and multipotent differentiation of mesenchymal stem cells from human fetal livers]
Xun-Zhuan Zhao1, Lai Wei, Mei Han
1Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
Objective:
To isolate and culture mesenchymal stem cells (MSCs) from human fetal livers and describe their biological characteristics.
Methods:
MSCs were acquired using an optimized method. Cell cycles and the immunophenotype of the cells were analyzed by flow cytometry. The osteogenic and adipogenic differentiations were induced and identified by specific stainings, and hepatic differentiation by morphology and RT-PCR.
Results:
The target cells derived from human fetal livers adhered to the plate with fibroblast-like morphology, whose surface markers were CD90, CD44, CD147 positive, and CD34, CD45, HLA-DR negtive. In the differentiation study, these cells could be induced to differentiate into osteogenic, adipogenic and hepatocyte-like cells.
Conclusion:
Multipotent MSCs can be isolated and cultured from human fetal livers.
