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Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export

Francisco Estruch1, Christine A Hodge, Susana Rodríguez-Navarro

  • 1Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universitat de Valencia, Dr. Moliner, 50. Burjassot 46100, Spain. francisco.estruch@uv.es

The Journal of Biological Chemistry
|December 28, 2004
PubMed

Insights

Zds1p, a protein interacting with Dbp5p and Gfd1p, is crucial for mRNA export. Deleting ZDS1 worsens export defects, suggesting Zds1p

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic gene expression relies on mRNA export from the nucleus.
  • Dbp5p, a DEAD box protein, is a vital, conserved mRNA export factor.
  • Dbp5p associates with nuclear pore complex (NPC) cytoplasmic filaments.

Purpose of the Study:

  • To investigate the role of Zds1p in mRNA export.
  • To determine the interaction of Zds1p with Dbp5p and Gfd1p.
  • To elucidate Zds1p's function in the mRNA export pathway.

Main Methods:

  • Yeast two-hybrid system for in vivo interaction studies.
  • In vitro binding assays to confirm direct protein interactions.
  • Genetic interaction analysis using mutant alleles.
  • Phenotypic analysis of gene deletion mutants in poly(A)+ export.

Main Results:

  • Zds1p interacts both in vivo and in vitro with Dbp5p and Gfd1p.
  • Gfd1p and Dbp5p directly bind to the C-terminal region of Zds1p.
  • ZDS1 shows genetic interactions with key mRNA export factors (DBP5, MEX67).
  • Deletion of ZDS1 or ZDS2 exacerbates mRNA export defects in dbp5 and mex67 mutants.

Conclusions:

  • Zds1p likely associates with the Dbp5p-Gfd1p-nucleoporin complex at NPC cytosolic fibrils.
  • Zds1p is essential for optimal mRNA export in yeast.
  • This study identifies Zds1p as a novel component of the mRNA export machinery.

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