Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

D cyclins in lymphocytes.

David Kaplan1, Howard Meyerson, William Husel

  • 1Department of Pathology, Case School of Medicine, Cleveland, Ohio 44106-4943, USA. drk5@cwru.edu

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|December 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human Herpesvirus-6A and -6B (HHV-6A and HHV-6B): The Role of Roseoloviruses in Neurological Dysfunction and the Mechanisms of Viral-Induced Epileptogenesis.

Viruses·2026
Same author

Nonlinear Bivariate Associations and Mononuclear Cell-Type-Specific Expression Level Differences in the STING Signalling Pathway.

Journal of cellular and molecular medicine·2026
Same author

Differential survival benefit of curative versus non-curative intent treatment in a real-world cohort with early and intermediate-stage hepatocellular carcinoma.

Hepatology communications·2026
Same author

Shaping the Future of Biomaterials through Contributions by Our Early Career Board Members.

ACS biomaterials science & engineering·2026
Same author

Elevating an invisible role: co-designing solutions to optimize medical office assistants in primary care.

BMC primary care·2026
Same author

Commentary on Journal of School Psychology Special Issue: Bayesian Statistics.

Journal of school psychology·2025
Same journal

A Modular High-Parameter Flow Cytometry Framework: Pre-Analytical Optimization and Validation for Clinical Research.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Quantitative Detection of Entotic Cell-In-Cell Structures Using Deformable Segmentation and Deep Learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Comparison of Tissue Preparations to Identify and Phenotype T Cells in Human Colorectal Tumor Tissue.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

Ensembling Unets for Rare Chromosomal Aberration Detection in Metaphase Images, Uncertainty Quantification, and Ionizing Radiation Dose Estimation.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same journal

OMIP-121: Immune Phenotyping of Canine Peripheral Leukocytes by Mass Cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
See all related articles

This study found that all three D cyclins, including cyclin D1, are present in human lymphocytes. This challenges the previous understanding of cyclin D1 tissue-specific expression and cell cycle regulation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Immunology

Background:

  • D-type cyclins regulate cell cycle progression through the G1 phase.
  • Previous research indicated D-type cyclins were not expressed in lymphocytes, with cyclins D2 and D3 being the primary forms found.

Purpose of the Study:

  • To re-evaluate D-cyclin expression in human lymphocytes using advanced techniques.
  • To investigate the presence and function of cyclin D1 in lymphocytes.

Main Methods:

  • High-resolution enzymatic amplification staining.
  • Flow cytometry and confocal microscopy.
  • Immunoblotting techniques.

Main Results:

  • All three D-cyclins, including cyclin D1, were detected in quiescent human peripheral blood lymphocytes.

Related Experiment Videos

  • Cyclin D1 was found complexed with CDK4, indicating functionality.
  • Cyclin D1 expression was observed in both cytoplasm (quiescent) and nucleus (activated) cells.

    • Conclusions:

    • Lymphocytes express cyclin D1, contradicting prior assumptions.
    • The hypothesis of redundant activities and tissue-specific expression of D-cyclins requires re-evaluation.