Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic mapping and ENU mutagenesis.

David R Beier1, Bruce J Herron

  • 1Genetics Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. beier@receptor.med.harvard.edu

Genetica
|December 29, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Sensitized ENU Mutagenesis Screen for Thrombosis Modifiers Identifies Suppressor Variants in Non-mutagenized Parental Generations Due to Antithrombotic Selective Pressures.

bioRxiv : the preprint server for biology·2026
Same author

The response to kidney injury is epigenetically regulated through the activation of bivalent genes.

American journal of physiology. Renal physiology·2026
Same author

A systems genetics approach identifies roles for proteasome factors in heart development and congenital heart defects.

PLoS genetics·2025
Same author

Centriolar protein PIBF1 is required for craniofacial and forebrain development.

Developmental biology·2025
Same author

Inhibition of Hedgehog signaling does not mitigate polycystic kidney disease severity in a Pkd1 mutant mouse model.

Journal of cell science·2025
Same author

A conditional smoothened (smo) allele on an inbred C57BL/6J genetic background has a hypomorphic smo mutant phenotype.

Developmental biology·2024
Same journal

Genomic insights into marine-freshwater transition: evolutionary adaptations in freshwater Pusa seals.

Genetica·2026
Same journal

Morphological and COI-based identification of species of the Hawkmoth genus Theretra Hübner, 1819 (Lepidoptera: Sphingidae) from Himachal Pradesh, India.

Genetica·2026
Same journal

Advancing genetics and evolution: new Editors-in-Chief in Genetica.

Genetica·2026
Same journal

Origin and diversification of Altai osmans (Oreoleuciscus), Far Eastern phoxinin minnows (Rhynchocypris) and Far Eastern redfins (Tribolodon) of the Leuciscidae family (Actinopterygii): an example of evolutionary tempo variation?

Genetica·2026
Same journal

Chloroplast genome comparison, phylogeny, and molecular evolution of five endemic Potentilla (Rosaceae) species in Mongolia.

Genetica·2026
Same journal

Severe trauma and chronic stress: seeds and fruits of epigenetic sensitivity.

Genetica·2026
See all related articles

Ethylnitrosourea (ENU) mutagenesis generates mouse mutations, but characterization is challenging. Interval haplotype analysis quickly maps these mutations, even with few affected offspring, streamlining genetic research.

Area of Science:

  • Genetics
  • Genomics
  • Mouse Models

Background:

  • Ethylnitrosourea (ENU) mutagenesis is a powerful tool for creating novel mutations in mice.
  • Investigating and characterizing these induced mutations presents significant logistical challenges.
  • Early determination of a mutation's map position is crucial for efficient downstream analysis.

Purpose of the Study:

  • To present interval haplotype analysis as a method for rapid mutation mapping in mice.
  • To demonstrate the utility of this approach even with limited numbers of affected progeny.

Main Methods:

  • Utilizing interval haplotype analysis.
  • Analyzing genetic data from affected mouse progeny.

Main Results:

Related Experiment Videos

  • Interval haplotype analysis effectively facilitates the determination of mutation map positions.
  • The method is efficient and applicable even with small sample sizes of affected offspring.

Conclusions:

  • Interval haplotype analysis is a valuable tool for accelerating the characterization of ENU-induced mutations in mice.
  • This approach significantly reduces the logistical demands of genetic studies involving novel mutations.