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[Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum]
Ming-shu Xu1, Lu-qi Huang, Mei-lan Chen
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
Objective:
To study the effect of several factors on the quantity of hypericin in H. perforatum callus.
Method:
High efficiency liquid phase chromatography and plant tissue culture were applied.
Result And Conclusion:
When the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.
