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[Study on the production condition of extraction in combination hydrolysis in situ for isolating diosgenin]
Jun Wang1, Jun Chen, Ke-di Yang
1School of Biological and Environmental Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China. wangjunjs@tom.com
Objective:
To study the production technology of extraction in combination hydrolysis in situ for isolating diosgenin from Dioscorea nipponica by orthogonal design.
Method:
The optimum production conditions were investigated with the recovery of diosgenin as an index by extraction in combination hydrolysis in situ, and were compared with the traditional method.
Result:
Extraction in combination hydrolysis in situ conducted in 1.5 mol x L(-1) sulfuric acid of water containing 75% isopropanol at 100 degrees C for 4.5 h could get higher recovery of diosgenin than traditional methods.
Conclusion:
This production technology can get higher recovery of diosgenin, and it is simple, time and money saving.
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