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Orexin-1 receptor expression after global ischemia in mice
Tomoya Nakamachi1, Sakura Endo, Hirokazu Ohtaki
1Department of Anatomy, Showa University School of Medicine, Shinagawa-Ku, Tokyo 142-8555, Japan.
Regulatory Peptides
|December 29, 2004
Summary
Orexin-1 receptors (OX1R) increase in the mouse brain after ischemic stroke, appearing in neurons and glial cells. This suggests orexins and OX1R play a role in the brain
Area of Science:
- Neuroscience
- Neurobiology
- Ischemic Stroke Research
Background:
- Orexins regulate feeding and sleep-wake cycles.
- Decreased orexin A in cerebrospinal fluid is linked to neurodegenerative diseases.
- Orexin receptor expression and localization in the central nervous system are not well understood.
Purpose of the Study:
- Investigate time-dependent changes in orexin-1 receptor (OX1R) expression and localization in the mouse brain.
- Examine OX1R cellular localization after transient common carotid artery occlusion (tCCAO).
Main Methods:
- Utilized immunohistochemical techniques in a mouse model of transient common carotid artery occlusion (tCCAO).
- Performed double-immunohistochemistry to identify OX1R co-localization with neuronal and glial markers.
Main Results:
- OX1R immunoreactivity significantly increased in the hippocampus and cortex 2 days post-tCCAO, peaking at this time.
- OX1R levels remained unchanged in the hypothalamus.
- OX1R-positive cells were found in neurons (NeuN+), astrocytes (GFAP+), and oligodendrocytes (CNPase+) 2 days after tCCAO.
Conclusions:
- OX1R is induced in non-neuronal cells, including astrocytes and oligodendrocytes, during ischemic stress.
- Orexins and their receptors may play a significant role in the response to ischemic insults.
- Findings highlight the involvement of the orexin system in the brain's reaction to ischemia.