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Updated: Aug 20, 2026

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
Inhibitory properties of nucleic acid-binding ligands on protein synthesis
Abba Malina1, Shakila Khan, Coby B Carlson
1Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, Que., Canada H3G 1Y6.
Abstract:
The use of small molecule inhibitors in the study of cellular processes is a powerful approach to understanding gene function. During the course of a high throughput screen for novel inhibitors of eukaryotic translation, we identified a number of nucleic acid binding ligands that showed activity in our assay. When tested on a panel of mRNA transcripts displaying different modes of translation initiation, these ligands showed a range of biological activities--with some inhibiting both cap-dependent and internal initiation and others preferentially blocking internal initiation. We used this information to identify a novel threading intercalator that inhibits Hepatitis C virus internal initiation.
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