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Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 11, 2010
Study of mRNA expression of Arabidopsis thaliana under stimulation using modified differential display RT-PCR with
Wang Bochu1, Zhang Jin, Li Biao
1Key Lab for Biomechanics and Tissue Engineering under the State Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China. wangbc2000@126.com
Abstract:
We studied differential expressions of Arabidopsis thaliana seedlings under sound stimulation using modified differential display RT-PCR with silver staining in this paper. Six differentially expressed cDNA fragments were obtained. Molecular weight of fragments was between 200 and 600 bp, respectively. On the base of the experimentation, we considered sound stress would have been a positive or negative influence on plant growth. The experimental results indicated some genes was activated, meanwhile some were restrained under after stimulation under sound stress.

