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Updated: Aug 20, 2026

Assaying the Kinase Activity of LRRK2 in vitro
Published on: January 18, 2012
Effect of leonurine on the activity of creatine kinase
Zhao Wang1, Pei-Lin Zhang, Yong Ju
1Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China. zwang@tsinghua.edu.cn
Abstract:
The effects of leonurine (1) on the activity of creatine kinase (CK) have been studied. The results show that leonurine inhibits enzyme activity in concentration- and time-dependent manners (at 0.75 and 1.51 mmol from 12 to 72 h). There are two mechanisms for the inhibition process. Compound 1 first acts as a non-competitive inhibitor and then as an irreversible inhibitor. Changes of CK were not found in 10% SDS-PAGE, but the amount of dimeric CK decreased in 10% non-SDS gel. The results suggest that 1 can inhibit CK activity by degrading its dimeric structure.
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