Related Experiment Videos
Influence of an orally effective SOD on hyperbaric oxygen-related cell damage
Claus M Muth1, Yvonne Glenz, Michael Klaus
1Sektion Anästhesiologische Pathophysiologie und Verfahrensentwicklung, Universitätsklinikum Ulm, D-89073 Ulm, Germany. claus-martin.muth@medizin.uni-ulm.de
Abstract:
In a prospective, double-blind, randomised placebo-controlled study, we tested the hypothesis that a new formulation consisting of wheat gliadin chemically combined with a vegetal (thus orally effective) preparation of superoxide dismutase (SOD) allows to prevent hyperbaric oxygen (HBO)-induced oxidative cell stress. Twenty healthy volunteers were exposed to 100% oxygen breathing at 2.5 ATA for a total of 60 min. DNA strand breaks (tail moments) were determined using the alkaline version of the comet assay. Whole blood concentrations of reduced (GSH) and oxidised (GSSG) glutathione and F2-isoprostanes, SOD, glutathione peroxidase (GPx) and catalase (Cat) activities and red cell malondialdehyde (MDA) content were determined. After HBO exposure the tail moment (p = 0.03) and isoprostane levels (p = 0.049) were significantly lower in the group that received the vegetal formulation. Neither SOD and Cat nor GSH and GSSG were significantly affected by this preparation or HBO exposure. By contrast, blood GPx activity, which tended to be lower in the SOD-group already before the HBO exposure (p = 0.076), was significantly lower afterwards (p = 0.045). We conclude that an orally effective SOD-wheat gliadin mixture is able to protect against DNA damage, which coincided with reduced blood isoprostane levels, and may therefore be used as an antioxidant.
Insights
A new superoxide dismutase (SOD) and wheat gliadin formulation protected against hyperbaric oxygen (HBO) induced DNA damage and reduced oxidative stress markers. This antioxidant may prevent cell damage from HBO exposure.
Area of Science:
- Biochemistry
- Cell Biology
- Oxidative Stress Research
Background:
- Hyperbaric oxygen (HBO) therapy can induce oxidative cell stress.
- Preventing HBO-induced oxidative stress is crucial for patient safety and treatment efficacy.
- Novel antioxidant formulations are needed to mitigate HBO-related cellular damage.
Purpose of the Study:
- To evaluate the efficacy of an orally effective formulation combining wheat gliadin and superoxide dismutase (SOD) in preventing hyperbaric oxygen (HBO)-induced oxidative cell stress.
- To assess the impact of this formulation on DNA damage and key oxidative stress biomarkers.
Main Methods:
- Prospective, double-blind, randomized, placebo-controlled study.
- Twenty healthy volunteers exposed to 100% oxygen at 2.5 ATA for 60 minutes.
- Assessed DNA strand breaks (comet assay), glutathione (GSH/GSSG), F2-isoprostanes, SOD, glutathione peroxidase (GPx), catalase (Cat), and malondialdehyde (MDA).
Main Results:
- The SOD-wheat gliadin group showed significantly lower DNA strand breaks (tail moment, p=0.03) and F2-isoprostane levels (p=0.049) after HBO exposure.
- No significant changes in SOD, catalase, GSH, or GSSG were observed.
- Blood GPx activity was significantly lower in the SOD group post-HBO exposure (p=0.045).
Conclusions:
- The orally effective SOD-wheat gliadin mixture demonstrates protective effects against HBO-induced DNA damage.
- Reduced blood isoprostane levels correlate with the observed DNA protection.
- This formulation shows potential as an antioxidant to counteract HBO-induced cellular stress.
Related Concept Videos
02:49Cancer Therapies
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
01:29Oxygen Requirements and Growth Patterns