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Fatty acid synthetase complex from the insect Ceratitis capitata.

A M Municio, M A Lizarbe, E Relaño

    Biochimica Et Biophysica Acta
    |April 26, 1977
    PubMed
    Summary

    Fatty acid synthesis in the Mediterranean fruit fly peaks during larval development. The isolated fatty acid synthetase complex produces various fatty acids, with palmitate as the primary product.

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    Area of Science:

    • Biochemistry
    • Insect Physiology

    Background:

    • Fatty acid synthesis is crucial for insect development and survival.
    • Understanding the enzymes involved provides insights into metabolic regulation.

    Purpose of the Study:

    • To investigate fatty acid synthesis capacity in Ceratitis capitata.
    • To characterize the fatty acid synthetase complex from this insect.

    Main Methods:

    • In vitro assays using [1-14C]acetyl-CoA with insect homogenates.
    • Isolation and purification of the fatty acid synthetase complex.
    • Enzyme characterization including molecular weight, subunit analysis, and kinetic properties.

    Main Results:

    • Maximum fatty acid synthesis activity observed in 5-6 day old larvae.
    • Purified fatty acid synthetase complex (MW 5.2x10^5) consists of subunits.
    • Enzyme activity depends on NADPH; FMN inhibits activity.
    • Synthesized fatty acids range from C10:0 to C18:0, predominantly palmitate.

    Conclusions:

    • Ceratitis capitata larvae possess significant fatty acid synthetic capacity.
    • The characterized fatty acid synthetase complex is a key enzyme in insect lipid metabolism.

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