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DRw antisera react with activated T cells.

W C DeWolf, S F Schlossman, E J Yunis

    Journal of Immunology (Baltimore, Md. : 1950)
    |May 1, 1979
    PubMed
    Summary
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    Activated T cells temporarily express specific determinants that react with certain HLA-DRw antisera. These T cell antigens reappear upon restimulation, suggesting unique subset reactivity.

    Area of Science:

    • Immunology
    • Cell Biology
    • Genetics

    Background:

    • T cells are crucial for immune responses.
    • Human Leukocyte Antigen (HLA) molecules play a role in immune recognition.
    • HLA-DRw antigens are involved in T cell activation and function.

    Purpose of the Study:

    • To investigate the expression of HLA-DRw determinants on activated T cells.
    • To understand the dynamics of these determinants during T cell activation and restimulation.
    • To explore the specificity of DRw alloantisera against activated T cells.

    Main Methods:

    • Nylon wool purification of T cells.
    • Lymphocytotoxicity assays using HLA-DRw antisera and complement.
    • Mixed lymphocyte culture (MLC) for T cell activation.

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  • Activation using mitogens like Concanavalin A (Con A) and purified protein-purified PHA (HA-17).

    • Main Results:

    • Resting T cells are nonreactive to HLA-DRw antisera.
    • Activated T cells transiently express DRw determinants, peaking at 6 days and declining by 16-18 days.
    • Restimulation or mitogenic activation (Con A, PHA) re-induces determinant expression.
    • Reactivity generally follows DRw genetic restriction but shows variability and occasional extra reactions depending on activation method.

    Conclusions:

    • Activated T cells express unique, transient determinants recognized by DRw alloantisera.
    • These determinants may represent novel allospecificities or Ia-like antigens on specific T cell subsets.
    • Further research is needed to clarify if DRw antisera target T cells, activation antigens, or differentiation antigens.