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[Preliminary study on oncogene MDM2 in acute leukemias]
1Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Objective:
To explore the causes of MDM2 gene overexpression.
Methods:
The amplification and overexpression of MDM2 gene in 41 acute leukemia patients and two leukemic cell lines (K051 and HL60) were studied by using Southern blot, dot hybridyzation and RT-PCR techniques.
Results:
Overexpression of MDM2 gene was found in 51.2% (21/41) of the patients and HL60 cell line, but no amplification of the gene was found in all the patients and the two cell lines.
Conclusion:
The level of MDM2 gene expression was related to the prognoses of the patients but not to FAB subtypes of acute leukemias.
