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Updated: Aug 20, 2026

Modeling Chemotherapy Resistant Leukemia In Vitro
Published on: February 9, 2016
[Biological characteristics of residual leukemic cells and their drug-resistant regrowth]
1State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Objective:
To elucidate the mechanisms of residual leukemia and its drug-resistant regrowth.
Methods:
Bone marrow cells from transplantable mouse leukemia models L615 and L7811 were cultivated in long-term culture system respectively. Daunorubicin (DNR) was added to the systems to mimick in vivo chemotherapy.
Results:
Residual leukemic cells on stromal layers escaped DNR killing. They derived from the adherent leukemic cell population, which was heterogeneous with many undifferentiated and dormant cells. They were drug-resistant and expressed bcl-2 gene.
Conclusion:
Bone marrow stromal layer was the vital microenvironment for the residual leukemic cells to survive and regrow, and might increase the drug-resistance by up-regulating bcl-2 gene expression.
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