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Updated: Aug 20, 2026

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
Published on: March 18, 2011
[Changes of erythrocyte membrane lipid in hereditary G6PD deficiency]
Objective:
To explore the biochemical mechanism of G6PD deficiency.
Methods:
A fluorescence polarization technique with 1,6-dipheny 1,1,3,5-heratciene (DPH) as a fluorescent probe was used to determine the fluidity of erythrocyte membrane lipid from G6PD deficient patients and normal controls. The polarization of fluorescence was measured between 0 degrees and 90 degrees.
Results:
The fluorescence polarization parameter (rho) was high in G6PD deficient erythrocytes as compared with normal controls, indicating a decrease in erythrocyte membrane lipid fluidity. The determination of lipoperoxidation of erythrocyte membrane by fluorescence spectrometry also showed that the content of MDA was significantly higher in G6PD deficient erythrocytes than in normal controls. The percentage of unsaturated fatty acid C18:1 and C18:2 in total phosphalipids and phosphatidyl serine was decreased in G6PD deficient erythrocytes.
Conclusion:
The decrease of erythrocyte membrane lipid fluidity is related to the lipid peroxidant, and the oxidative damage of erythrocyte membrane phosphalipids in G6PD deficient patients might be an important factor of hemolysis.
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